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ATG4A Recombinant Antibody, Biotin Conjugated

BSM-61617R-BIOTIN
Bioss Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATG4A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ATG4A Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID115201
  • Target name
    ATG4A
  • Target description
    autophagy related 4A cysteine peptidase
  • Target synonyms
    APG4 autophagy 4 homolog A; APG4A; ATG4 autophagy related 4 homolog A; AUTL2; AUT-like 2, cysteine endopeptidase; autophagin 2; autophagy-related cysteine endopeptidase 2; autophagy-related protein 4 homolog A; cysteine protease ATG4A; hAPG4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYN0
  • Protein Name
    Cysteine protease ATG4A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG4A using anti-ATG4A antibody (A06539-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4A antigen affinity purified polyclonal antibody (Catalog # A06539-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4A at approximately 50KD. The expected band size for ATG4A is at 45KD.
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