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Western Blot Positive WB detected in: Mouse brain tissue All lanes: ATG4B antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 53, 32, 38, 43 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: ATG4B antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 53, 32, 38, 43 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: ATG4B antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 53, 32, 38, 43 kDa Observed band size: 45 kDa

ATG4B Antibody

CSB-PA002290LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetATG4B
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATG4B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23192
  • Target name
    ATG4B
  • Target description
    autophagy related 4B cysteine peptidase
  • Target synonyms
    APG4B, AUTL1, HsAPG4B, cysteine protease ATG4B, APG4 autophagy 4 homolog B, ATG4 autophagy related 4 homolog B, AUT-like 1 cysteine endopeptidase, autophagin-1, autophagy-related cysteine endopeptidase 1, autophagy-related protein 4 homolog B, hAPG4B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4P1
  • Protein Name
    Cysteine protease ATG4B
  • Scientific Description
    Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATG4B using anti-ATG4B antibody (A02885-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4B antigen affinity purified polyclonal antibody (Catalog # A02885-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4B at approximately 48 kDa. The expected band size for ATG4B is at 48 kDa.
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