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Atg4C Polyclonal Antibody

CAC10660
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATG4C
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Overview

  • Supplier
    Biomatik
  • Product Name
    ATG4C Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84938
  • Target name
    ATG4C
  • Target description
    autophagy related 4C cysteine peptidase
  • Target synonyms
    APG4-C, APG4C, AUTL1, AUTL3, HsAPG4C, cysteine protease ATG4C, APG4 autophagy 4 homolog C, ATG4 autophagy related 4 homolog C, AUT-like 1, cysteine endopeptidase, AUT-like 3 cysteine endopeptidase, autophagin-3, autophagy-related cysteine endopeptidase 3, autophagy-related protein 4 homolog C, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DT6
  • Protein Name
    Cysteine protease ATG4C
  • Scientific Description
    The Atg4C Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG4C using anti-ATG4C antibody (A09728-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4C antigen affinity purified polyclonal antibody (Catalog # A09728-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4C at approximately 52 kDa. The expected band size for ATG4C is at 52 kDa.
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