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Anti-ATOH1 Antibody

CAB6530
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetATOH1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-ATOH1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID474
  • Target name
    ATOH1
  • Target description
    atonal bHLH transcription factor 1
  • Target synonyms
    ATH1, DFNA89, HATH1, MATH-1, bHLHa14, transcription factor ATOH1, atonal homolog 1, atonal homolog bHLH transcription factor 1, basic helix-loop-helix family member a14, class A basic helix-loop-helix protein 14, helix-loop-helix protein hATH-1, protein atonal homolog 1
  • Host
    Rabbit
  • Protein IDQ92858
  • Protein Name
    Transcription factor ATOH1
  • Scientific Description
    ATOH1 Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MATH1/HATH1/ATOH1 using anti-MATH1/HATH1/ATOH1 antibody (A02207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATH1/HATH1/ATOH1 antigen affinity purified polyclonal antibody (Catalog # A02207-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATH1/HATH1/ATOH1 at approximately 38 kDa. The expected band size for MATH1/HATH1/ATOH1 is at 38 kDa.
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