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ATP11C Antibody (aa589-616)

LS-C156636
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATP11C
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATP11C Antibody (aa589-616)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:10 - 1:50), IHC, IHC-P (1:50 - 1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID286410
  • Target name
    ATP11C
  • Target description
    ATPase phospholipid transporting 11C (ATP11C blood group)
  • Target synonyms
    ATPIG, ATPIQ, HACXL, phospholipid-transporting ATPase IG, ATPase, class VI, type 11C, P4-ATPase flippase complex alpha subunit ATP11C, probable phospholipid-transporting ATPase IG
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP11C using anti-ATP11C antibody (A06516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP11C antigen affinity purified polyclonal antibody (Catalog # A06516-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP11C at approximately 129 kDa. The expected band size for ATP11C is at 129 kDa.
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ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetATP11C
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