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ATP1A2 Antibody

CSB-PA020068
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetATP1A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATP1A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID477
  • Target name
    ATP1A2
  • Target description
    ATPase Na+/K+ transporting subunit alpha 2
  • Target synonyms
    ATPase Na+/K+ transporting alpha 2 polypeptide; FHM2; MHP2; Na(+)/K(+) ATPase alpha-2 subunit; Na+/K+ ATPase, alpha-A(+) catalytic polypeptide; Na+/K+ ATPase, alpha-B polypeptide; sodium pump subunit alpha-2; sodium/potassium-transporting ATPase alpha-2 chain; sodium/potassium-transporting ATPase subunit alpha-2; sodium-potassium ATPase catalytic subunit alpha-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50993
  • Protein Name
    Sodium/potassium-transporting ATPase subunit alpha-2
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATP1A2 using anti-ATP1A2 antibody (A02064-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,(100°C boiled for 5 minutes) Lane 2: rat brain tissue lysates,(37°C incubated for 10 minutes) Lane 3: mouse brain tissue lysates,(100°C boiled for 5 minutes) Lane 4: mouse brain tissue lysates.(37°C incubated for 10 minutes) After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A2 antigen affinity purified polyclonal antibody (Catalog # A02064-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A2 at approximately 112 kDa. The expected band size for ATP1A2 is at 112 kDa.
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