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ATP1A4 Antibody (N-Terminus)

LS-C757999
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetATP1A4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATP1A4 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Western Blot Predicted Molecular Weight: 114.2 kDa
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    ELISA, Flo (1:25), WB (1:2000) Western Blot Predicted Molecular Weight: 114.2 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID480
  • Target name
    ATP1A4
  • Target description
    ATPase Na+/K+ transporting subunit alpha 4
  • Target synonyms
    ATP1A1, ATP1AL2, sodium/potassium-transporting ATPase subunit alpha-4, ATPase, Na+/K+ transporting, alpha 4 polypeptide, ATPase, Na+/K+ transporting, alpha polypeptide-like 2, Na(+)/K(+) ATPase alpha-4 subunit, Na+/K+ ATPase 4, Na+/K+ ATPase, alpha-D polypeptide, Na,K-ATPase subunit alpha-C, sodium pump 4, sodium pump subunit alpha-4, sodium-potassium ATPase catalytic subunit alpha-4, sodium/potassium-transporting ATPase alpha-4 chain
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP1A4 using anti-ATP1A4 antibody (A09005). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A4 antigen affinity purified polyclonal antibody (Catalog # A09005) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A4 at approximately 100 kDa. The expected band size for ATP1A4 is at 114 kDa.
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