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Western Blot analysis of Jurkat cells using ATP5H Polyclonal Antibody
Western Blot analysis of Jurkat cells using ATP5H Polyclonal Antibody
Western Blot analysis of Jurkat cells using ATP5H Polyclonal Antibody

ATP5H Antibody

CSB-PA000953
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP5PD
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATP5H Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10476
  • Target name
    ATP5PD
  • Target description
    ATP synthase peripheral stalk subunit d
  • Target synonyms
    APT5H, ATP5H, ATPQ, ATP synthase peripheral stalk subunit d, mitochondrial, ATP synthase D chain, mitochondrial, ATP synthase subunit d, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, ATP synthase, H+ transporting, mitochondrial F1F0, subunit d, ATP synthase, H+ transporting, mitochondrial Fo complex subunit D, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d, ATPase subunit d, My032 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75947
  • Protein Name
    ATP synthase subunit d, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATP5H using anti-ATP5H antibody (PB9328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5H antigen affinity purified polyclonal antibody (Catalog # PB9328) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22 kDa. The expected band size for ATP5H is at 22 kDa.
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