![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640318/GTX640318_T-45397_20240510_WB_M_R_24051400_322.webp)
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATP5J antibody [HL2934]
GTX640318
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATP5PF
Overview
- SupplierGeneTex
- Product NameATP5J antibody [HL2934]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2934
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID522
- Target nameATP5PF
- Target descriptionATP synthase peripheral stalk subunit F6
- Target synonymsATP5, ATP5A, ATP5J, ATPM, CF6, F6, ATP synthase peripheral stalk subunit F6, mitochondrial, ATP synthase subunit h, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6, ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, coupling factor 6, mitochondrial ATP synthase, coupling factor 6, mitochondrial ATP synthase, subunit F6, mitochondrial ATPase coupling factor 6, proliferation-inducing protein 36
- HostRabbit
- IsotypeIgG
- Protein IDP18859
- Protein NameATP synthase peripheral stalk subunit F6, mitochondrial
- Scientific DescriptionMitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. ATP5J stained by ATP5J antibody [HL2934] (GTX640318) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. ATP5J stained by ATP5J antibody [HL2934] (GTX640318) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640318/GTX640318_T-45397_20240619_IHC-P_1_24062501_661.webp)
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody [HL2934] (GTX640318) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640318/GTX640318_45453_20240628_WB_24070822_733.webp)
![ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis. Sample: Paraffin-embedded human heart. ATP5J stained by ATP5J antibody [HL2934] (GTX640318) diluted at 1:200. Antigen Retrieval: Tris-EDTA-Citrate buffer, pH 7.8, 15 min ATP5J antibody [HL2934] detects ATP5J protein by immunohistochemical analysis. Sample: Paraffin-embedded human heart. ATP5J stained by ATP5J antibody [HL2934] (GTX640318) diluted at 1:200. Antigen Retrieval: Tris-EDTA-Citrate buffer, pH 7.8, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640318/GTX640318_45761_20250529_IHC-P_25061003_824.webp)





