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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA258902(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA258902(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA258902(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)

AUP1 Antibody

CSB-PA258902
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAUP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    AUP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID550
  • Target name
    AUP1
  • Target description
    AUP1 lipid droplet regulating VLDL assembly factor
  • Target synonyms
    ancient ubiquitous protein 1; lipid droplet-regulating VLDL assembly factor AUP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y679
  • Protein Name
    Ancient ubiquitous protein 1
  • Scientific Description
    This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AUP1 using anti-AUP1 antibody (A08937-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AUP1 antigen affinity purified polyclonal antibody (Catalog # A08937-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AUP1 at approximately 42 kDa. The expected band size for AUP1 is at 42 kDa.
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