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WB analysis of NIH-3T3 lysate using GTX89114 AVPR1A antibody, Internal. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of NIH-3T3 lysate using GTX89114 AVPR1A antibody, Internal. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of NIH-3T3 lysate using GTX89114 AVPR1A antibody, Internal. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer

AVPR1A antibody, Internal

GTX89114
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rodent, Rat
TargetAVPR1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    AVPR1A antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.03-0.1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID552
  • Target name
    AVPR1A
  • Target description
    arginine vasopressin receptor 1A
  • Target synonyms
    AVPR V1a, AVPR1, V1aR, vasopressin V1a receptor, SCCL vasopressin subtype 1a receptor, V1-vascular vasopressin receptor AVPR1A, V1a vasopressin receptor, antidiuretic hormone receptor 1A, vascular/hepatic-type arginine vasopressin receptor
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP37288
  • Protein Name
    Vasopressin V1a receptor
  • Scientific Description
    The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rodent, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hou W, Ma H, Xun Y, et al. Sex-Dependent Effects of Chronic Social Defeat on Emotional and Social Behaviors, and Parameters of Oxytocin and Vasopressin Systems in Mandarin Voles (Microtus mandarinus). Front Neurosci. 2021,15:625116. doi: 10.3389/fnins.2021.625116
    Read this paper
  • Yuan W, Li L, Hou W, et al. Preweaning Paternal Deprivation Impacts Parental Responses to Pups and Alters the Serum Oxytocin and Corticosterone Levels and Oxytocin Receptor, Vasopressin 1A Receptor, Oestrogen Receptor, Dopamine Type I Receptor, Dopamine Type II Receptor Levels in Relevant Brain Regions in Adult Mandarin Voles. Neuroendocrinology. 2020,110(3-4):292-306. doi: 10.1159/000501798
    Read this paper
  • He Z, Young L, Ma XM, et al. Increased anxiety and decreased sociability induced by paternal deprivation involve the PVN-PrL OTergic pathway. Elife. 2019,8. doi: 10.7554/eLife.44026
    Read this paper

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Figure 1. Western blot analysis of AVPR1A using anti-AVPR1A antibody (A03279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AVPR1A antigen affinity purified polyclonal antibody (Catalog # A03279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AVPR1A at approximately 60KD. The expected band size for AVPR1A is at 47KD.
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