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ProductsBack/AVPR1A Rabbit Polyclonal Antibody
WB Suggested Anti-AVPR1A Antibody Titration: 1.0 ug/ml Positive Control: 293T Whole Cell
WB Suggested Anti-AVPR1A Antibody Titration: 1.0 ug/ml Positive Control: 293T Whole Cell
WB Suggested Anti-AVPR1A Antibody Titration: 1.0 ug/ml Positive Control: 293T Whole Cell

AVPR1A Rabbit Polyclonal Antibody

TA358317
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetAVPR1A
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    AVPR1A Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID552
  • Target name
    AVPR1A
  • Target description
    arginine vasopressin receptor 1A
  • Target synonyms
    antidiuretic hormone receptor 1A; AVPR V1a; AVPR1; SCCL vasopressin subtype 1a receptor; V1a vasopressin receptor; V1aR; V1-vascular vasopressin receptor AVPR1A; vascular/hepatic-type arginine vasopressin receptor; vasopressin V1a receptor
  • Host
    Rabbit
  • Protein IDP37288
  • Protein Name
    Vasopressin V1a receptor
  • Scientific Description
    AVPR1A Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA002468LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of AVPR1A using anti-AVPR1A antibody (A03279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AVPR1A antigen affinity purified polyclonal antibody (Catalog # A03279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AVPR1A at approximately 60KD. The expected band size for AVPR1A is at 47KD.
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