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ProductsBack/AXL antibody [7E10]
ICC/IF analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using GTX83125 AXL antibody [7E10]. Green : AXL Blue: DRAQ5 fluorescent DNA dye
ICC/IF analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using GTX83125 AXL antibody [7E10]. Green : AXL Blue: DRAQ5 fluorescent DNA dye
ICC/IF analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using GTX83125 AXL antibody [7E10]. Green : AXL Blue: DRAQ5 fluorescent DNA dye

AXL antibody [7E10]

GTX83125
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetAXL
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Overview

  • Supplier
    GeneTex
  • Product Name
    AXL antibody [7E10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID558
  • Target name
    AXL
  • Target description
    AXL receptor tyrosine kinase
  • Target synonyms
    ARK; AXL oncogene; AXL transforming sequence/gene; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP30530
  • Protein Name
    Tyrosine-protein kinase receptor UFO
  • Scientific Description
    The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AXL using anti-AXL antibody (A00226-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXL antigen affinity purified polyclonal antibody (Catalog # A00226-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXL at approximately 140KD. The expected band size for AXL is at 98KD.
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