Azide-PEG3-FLAG, Azide-PEG3-FLAGtag (DYKDDDDK)

Jena Bioscience

Azide-PEG3-FLAG, Azide-PEG3-FLAGtag (DYKDDDDK)

5

Research Use Only

≥95%

C59H88N16O27S

1485.49 g/mol

Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 microM)[1]. This concentrations may serve as a starting point for individual assay set-up.

-20°C

12352200