Bio-Connect

Azide-PEG3-FLAG, Azide-PEG3-FLAGtag (DYKDDDDK)

Research Use Only
CLK-032-S
Jena Bioscience
Estimated Purity≥95%
Product group Chemicals
Molecular Weight1485.49 g/mol
€ 267,00
0.5 umol
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Jena Bioscience
  • Product Name
    Azide-PEG3-FLAG, Azide-PEG3-FLAGtag (DYKDDDDK)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Estimated Purity
    ≥95%
  • Molecular Formula
    C59H88N16O27S
  • Molecular Weight
    1485.49 g/mol
  • Scientific Description
    Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 microM)[1]. This concentrations may serve as a starting point for individual assay set-up.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352200