Azide-PEG4-aminoallyl-dUTP

Jena Bioscience

Azide-PEG4-aminoallyl-dUTP

5

Research Use Only

Azide-PEG4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasksto introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for subsequent microscopic imagingto crosslink the DNA to Azide- or DBCO-functionalized biomolecules e.g.proteins

-20°C

41116133