B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913)
10% SDS-PAGE
The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100913/GTX100913_39471_IP_w_23060100_499.webp "B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913)
10% SDS-PAGE
The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100913/GTX100913_43607_20190628_WB_w_23060100_350.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100913/GTX100913_39471_20151029_WB_B_w_23060100_427.webp "B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.")
![B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100913/GTX100913_43607_20210129_IHC-P_w_23060100_386.webp "B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. A. 30 μg PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000](https://www.genetex.com/upload/website/prouct_img/normal/GTX100913/GTX100913_39471_WB_R_w_23060100_812.webp "B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. A. 30 μg PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000")
B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
B-Raf antibody [N2C1], Internal
GTX100913
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBRAF
Overview
- SupplierGeneTex
- Product NameB-Raf antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.21 mg/ml
- ConjugateUnconjugated
- Gene ID673
- Target nameBRAF
- Target descriptionB-Raf proto-oncogene, serine/threonine kinase
- Target synonymsB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1, serine/threonine-protein kinase B-raf, 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), B-Raf serine/threonine-protein, murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, v-raf murine sarcoma viral oncogene homolog B, v-raf murine sarcoma viral oncogene homolog B1
- HostRabbit
- IsotypeIgG
- Protein IDP15056
- Protein NameSerine/threonine-protein kinase B-raf
- Scientific DescriptionThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Tsuji S, Kohyanagi N, Mizuno T, et al. Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A. Oncol Lett. 2021,21(2):113. doi: 10.3892/ol.2020.12374Read this paper
- Tateishi K, Ikegaya N, Udaka N, et al. BRAF V600E mutation mediates FDG-methionine uptake mismatch in polymorphous low-grade neuroepithelial tumor of the young. Acta Neuropathol Commun. 2020,8(1):139. doi: 10.1186/s40478-020-01023-3Read this paper
- Sase H, Nakanishi Y, Aida S, et al. Acquired JHDM1D-BRAF Fusion Confers Resistance to FGFR Inhibition in FGFR2-Amplified Gastric Cancer. Mol Cancer Ther. 2018,17(10):2217-2225. doi: 10.1158/1535-7163.MCT-17-1022Read this paper
- Rubin B, Monticelli H, Redaelli M, et al. Mitogen-Activated Protein Kinase Pathway: Genetic Analysis of 95 Adrenocortical Tumors. Cancer Invest. 2015,33(10):526-31. doi: 10.3109/07357907.2015.1080832Read this paper
Datasheet
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