HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BRAF (Cat# RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# TA500431). Positive lysates [LY401382] (100ug) and [LC401382] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BRAF (Cat# RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# TA500431). Positive lysates [LY401382] (100ug) and [LC401382] (20ug) can be purchased separately from OriGene.
B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI5A9]
CF500431
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetBRAF
Overview
- SupplierOriGene
- Product NameB Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI5A9]
- Delivery Days Customer14
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDOTI5A9
- Gene ID673
- Target nameBRAF
- Target descriptionB-Raf proto-oncogene, serine/threonine kinase
- Target synonymsB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1, serine/threonine-protein kinase B-raf, 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), B-Raf serine/threonine-protein, murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, v-raf murine sarcoma viral oncogene homolog B, v-raf murine sarcoma viral oncogene homolog B1
- HostMouse
- IsotypeIgG1
- Protein IDP15056
- Protein NameSerine/threonine-protein kinase B-raf
- Scientific DescriptionCarrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
MSDS
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