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Western Blot Positive WB detected in: Mouse spleen tissue, Mouse stomach tissue All lanes: B2m antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
Western Blot Positive WB detected in: Mouse spleen tissue, Mouse stomach tissue All lanes: B2m antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
Western Blot Positive WB detected in: Mouse spleen tissue, Mouse stomach tissue All lanes: B2m antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa

B2m Antibody

CSB-PA002486LA01MO
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetB2m
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Overview

  • Supplier
    Cusabio
  • Product Name
    B2m Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID12010
  • Target name
    B2m
  • Target description
    beta-2 microglobulin
  • Target synonyms
    Ly-m11, beta2-m, beta2m, beta-2-microglobulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01887
  • Protein Name
    Beta-2-microglobulin
  • Scientific Description
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat small intestine tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta 2 Microglobulin/B2m antigen affinity purified polyclonal antibody (Catalog # A00456-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta 2 Microglobulin/B2m at approximately 13KD. The expected band size for Beta 2 Microglobulin/B2m is at 13KD.
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