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ProductsBack/B3GNT8 Antibody (aa360-397)

B3GNT8 Antibody (aa360-397)

LS-C407898
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetB3GNT8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    B3GNT8 Antibody (aa360-397)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml) IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID374907
  • Target name
    B3GNT8
  • Target description
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
  • Target synonyms
    B3GALT7, BGALT15, beta3Gn-T8, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8, BGnT-8, UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 7, beta galactosyltransferase BGALT15, beta-1,3-Gn-T8, beta-1,3-N-acetylglucosaminyltransferase 8, beta1,3-N-acetylglucosaminyltransferase 8
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of B3GNT8 using anti-B3GNT8 antibody (PB9686). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B3GNT8 antigen affinity purified polyclonal antibody (Catalog # PB9686) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B3GNT8 at approximately 43 kDa. The expected band size for B3GNT8 is at 43 kDa.
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