CSB-EAP731137 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for African swine fever virus Envelope protein p54 (Ba71V-126) has been pre-coated onto the microwells. The African swine fever virus Envelope protein p54 (Ba71V-126) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for African swine fever virus Envelope protein p54 (Ba71V-126) is added to detect the captured African swine fever virus Envelope protein p54 (Ba71V-126) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.