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ProductsBack/BACH1 Antibody (aa250-263)

BACH1 Antibody (aa250-263)

LS-C186445
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBACH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BACH1 Antibody (aa250-263)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80kD band observed in Human Spleen and Peripheral Blood Lymphocytes lysates (calculated MW of 82.0kD according to NP_001177.1). Recommended concentration: 1-3 ug/ml.. Peptide-ELISA (1:128000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80kD band observed in Human Spleen and Peripheral Blood Lymphocytes lysates (calculated MW of 82.0kD according to NP_001177.1). Recommended concentration: 1-3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID571
  • Target name
    BACH1
  • Target description
    BTB domain and CNC homolog 1
  • Target synonyms
    BACH-1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; BTBD24; transcription regulator protein BACH1
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BACH1.3/BACH1 using anti-BACH1.3/BACH1 antibody (A02347-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACH1.3/BACH1 antigen affinity purified polyclonal antibody (Catalog # A02347-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACH1.3/BACH1 at approximately 100 kDa. The expected band size for BACH1.3/BACH1 is at 100 kDa.
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