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Western Blot analysis of 293 cells using BACH1 Polyclonal Antibody
Western Blot analysis of 293 cells using BACH1 Polyclonal Antibody
Western Blot analysis of 293 cells using BACH1 Polyclonal Antibody

BACH1 Antibody

CSB-PA000965
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBACH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    BACH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID571
  • Target name
    BACH1
  • Target description
    BTB domain and CNC homolog 1
  • Target synonyms
    BACH-1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; BTBD24; transcription regulator protein BACH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14867
  • Protein Name
    Transcription regulator protein BACH1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BACH1.3/BACH1 using anti-BACH1.3/BACH1 antibody (A02347-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACH1.3/BACH1 antigen affinity purified polyclonal antibody (Catalog # A02347-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACH1.3/BACH1 at approximately 100 kDa. The expected band size for BACH1.3/BACH1 is at 100 kDa.
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