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ProductsBack/BAFF Receptor antibody [9B9] (Azide free)

BAFF Receptor antibody [9B9] (Azide free)

GTX33920-00
GeneTex
ApplicationsFlow Cytometry, Other Application
DownloadDatasheet
Product group Antibodies
TargetTnfrsf13c
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Overview

  • Supplier
    GeneTex
  • Product Name
    BAFF Receptor antibody [9B9] (Azide free)
  • Delivery Days Customer
    9
  • Application Supplier Note
    Depletion: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9B9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID72049
  • Target name
    Tnfrsf13c
  • Target description
    tumor necrosis factor receptor superfamily, member 13c
  • Target synonyms
    2010006P15Rik; b cell-activating factor receptor; Baf; BAFF; BAFF receptor; Baffr; BAFF-R; B-cell maturation defect 1; B-cell-activating factor receptor; Bcmd; Bcmd1; Bcmd-1; BLyS receptor 3; Lv; Lvis22; tumor necrosis factor receptor superfamily member 13C
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDQ9D8D0
  • Protein Name
    Tumor necrosis factor receptor superfamily member 13C
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19, 40KD. The expected band size for BAFF Receptor is at 19KD.
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