BAFF: TACI [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

BPS Bioscience

BAFF: TACI [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

23

Research Use Only

Biotin

The BAFF: TACI [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between BAFF (B-cell Activating Factor) and TACI (Transmembrane activator and CAML interactor, also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B)) for screening and profiling applications. This kit comes with enough purified BAFF (amino acids 134-285) and biotin-labeled TACI (amino acids 23-166), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. BAFF: TACI [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with BAFF protein. After coating and blocking, biotinylated TACI is added in an optimized assay buffer. Next, unbound biotinylated TACI is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to TACI binding to BAFF. Need us to run inhibitor screens or profile your compounds against BAFF:TACI? Check out our Immunotherapy Screening Services.

41116100

Human