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BAG1 Antibody (YA932, Mouse)

HY-P81259
MedChem Express
TargetBAG1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BAG1 Antibody (YA932, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID573
  • Target name
    BAG1
  • Target description
    BAG cochaperone 1
  • Target synonyms
    BAG-1, HAP, RAP46, BAG family molecular chaperone regulator 1, BCL2 associated athanogene 1, BCL2-associated athanogene, Bcl-2 associating athanogene-1 protein, Bcl-2-binding protein, glucocortoid receptor-associated protein RAP46, receptor-associated protein, 46-KD
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    BAG1 Antibody (YA932) is a non-conjugated IgG1 antibody, targeting BAG1, with a predicted molecular weight of 39 kDa (observed band size: 52,46,33 kDa). BAG1 Antibody (YA932) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
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