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ProductsBack/BAG1 Rabbit Polyclonal Antibody
WB Suggested Anti-BAG1 Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellBAG1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
WB Suggested Anti-BAG1 Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellBAG1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
WB Suggested Anti-BAG1 Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellBAG1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

BAG1 Rabbit Polyclonal Antibody

TA358441
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetBAG1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    BAG1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID573
  • Target name
    BAG1
  • Target description
    BAG cochaperone 1
  • Target synonyms
    BAG family molecular chaperone regulator 1; BAG-1; BCL2 associated athanogene 1; Bcl-2 associating athanogene-1 protein; BCL2-associated athanogene; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46; HAP; RAP46; receptor-associated protein, 46-KD
  • Host
    Rabbit
  • Protein IDQ99933
  • Protein Name
    BAG family molecular chaperone regulator 1
  • Scientific Description
    BAG1 Antibody - N-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
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