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Western Blot analysis of Jurkat K562 cells using BAGE5 Polyclonal Antibody
Western Blot analysis of Jurkat K562 cells using BAGE5 Polyclonal Antibody
Western Blot analysis of Jurkat K562 cells using BAGE5 Polyclonal Antibody

BAG5 Antibody

CSB-PA004680
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBAG5
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Overview

  • Supplier
    Cusabio
  • Product Name
    BAG5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9529
  • Target name
    BAG5
  • Target description
    BAG cochaperone 5
  • Target synonyms
    BAG-5, CMD2F, BAG family molecular chaperone regulator 5, BCL2 associated athanogene 5, bcl-2-associated athanogene 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UL15
  • Protein Name
    BAG family molecular chaperone regulator 5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Bag5 using anti-Bag5 antibody (A07402). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag5 antigen affinity purified polyclonal antibody (Catalog # A07402) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag5 at approximately 51 kDa. The expected band size for Bag5 is at 51 kDa.
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