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BAT3 Antibody (YA2251, Rabbit)

HY-P82506
MedChem Express
TargetBAG6
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BAT3 Antibody (YA2251, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7917
  • Target name
    BAG6
  • Target description
    BAG cochaperone 6
  • Target synonyms
    BAG family molecular chaperone regulator 6; BAG-6; BAT3; BCL2 associated athanogene 6; D6S52E; G3; HLA-B-associated transcript 3; large proline-rich protein BAG6; large proline-rich protein BAT3; protein G3; protein Scythe; scythe
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    BAT3 Antibody (YA2251) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2251), targeting BAT3, with a predicted molecular weight of 119 kDa (observed band size: 150 kDa). BAT3 Antibody (YA2251) can be used for WB, ICC/IF experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAT3/BAG6 antigen affinity purified polyclonal antibody (Catalog # A00967-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAT3/BAG6 at approximately 150 kDa. The expected band size for BAT3/BAG6 is at 119 kDa.
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