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BAZ1A / ACF1 Antibody

LS-C411382
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetBAZ1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BAZ1A / ACF1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 175kDa/178kDa, while the observed MW by Western blot was 250kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 175kDa/178kDa, while the observed MW by Western blot was 250kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11177
  • Target name
    BAZ1A
  • Target description
    bromodomain adjacent to zinc finger domain 1A
  • Target synonyms
    ACF1; ATP-dependent chromatin remodeling protein; ATP-utilizing chromatin assembly and remodeling factor 1; bromodomain adjacent to zinc finger domain protein 1A; CHRAC subunit ACF1; hACF1; hWALp1; WALp1; WCRF180; williams syndrome transcription factor-related chromatin-remodeling factor 180
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACF1/BAZ1A using anti-ACF1/BAZ1A antibody (A04090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACF1/BAZ1A antigen affinity purified polyclonal antibody (Catalog # A04090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACF1/BAZ1A at approximately 203 kDa. The expected band size for ACF1/BAZ1A is at 179 kDa.
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