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Western Blot analysis of 293T cells using BAP31 Polyclonal Antibody
Western Blot analysis of 293T cells using BAP31 Polyclonal Antibody
Western Blot analysis of 293T cells using BAP31 Polyclonal Antibody

BCAP31 Antibody

CSB-PA000973
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCAP31
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Overview

  • Supplier
    Cusabio
  • Product Name
    BCAP31 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10134
  • Target name
    BCAP31
  • Target description
    B cell receptor associated protein 31
  • Target synonyms
    6C6-AG; 6C6-AG tumor-associated antigen; BAP31; B-cell receptor-associated protein 31; BCR-associated protein Bap31; CDM; DDCH; DXS1357E; p28 Bap31
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51572
  • Protein Name
    B-cell receptor-associated protein 31
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BAP31/BCAP31 using anti-BAP31/BCAP31 antibody (A03767-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP31/BCAP31 antigen affinity purified polyclonal antibody (Catalog # A03767-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAP31/BCAP31 at approximately 28 kDa. The expected band size for BAP31/BCAP31 is at 28 kDa.
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