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BCAP31 Antibody (OAAB18691)

OAAB18691
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetBCAP31
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    BCAP31 Antibody (OAAB18691)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1502CT208.31.16
  • Format
    Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Gene ID10134
  • Target name
    BCAP31
  • Target description
    B cell receptor associated protein 31
  • Target synonyms
    6C6-AG; 6C6-AG tumor-associated antigen; BAP31; B-cell receptor-associated protein 31; BCR-associated protein Bap31; CDM; DDCH; DXS1357E; p28 Bap31
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BAP31/BCAP31 using anti-BAP31/BCAP31 antibody (A03767-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP31/BCAP31 antigen affinity purified polyclonal antibody (Catalog # A03767-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAP31/BCAP31 at approximately 28 kDa. The expected band size for BAP31/BCAP31 is at 28 kDa.
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