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WB analysis of human fetal small intestine tissue using GTX46480 BCAT1 antibody at 0.2-1microg/ml.
WB analysis of human fetal small intestine tissue using GTX46480 BCAT1 antibody at 0.2-1microg/ml.
WB analysis of human fetal small intestine tissue using GTX46480 BCAT1 antibody at 0.2-1microg/ml.

BCAT1 antibody, N-term

GTX46480
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetBCAT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    BCAT1 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID586
  • Target name
    BCAT1
  • Target description
    branched chain amino acid transaminase 1
  • Target synonyms
    BCATC, BCT1, ECA39, MECA39, PNAS121, PP18, branched-chain-amino-acid aminotransferase, cytosolic, branched chain amino-acid transaminase 1, cytosolic, branched chain aminotransferase 1, cytosolic, placental protein 18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54687
  • Protein Name
    Branched-chain-amino-acid aminotransferase, cytosolic
  • Scientific Description
    This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCAT1 using anti-BCAT1 antibody (A05089-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT1 antigen affinity purified polyclonal antibody (Catalog # A05089-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAT1 at approximately 43 kDa. The expected band size for BCAT1 is at 43 kDa.
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