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Western blot All lanes: BCAT2 antibody at 14microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 34 kDa Observed band size: 34 kDa
Western blot All lanes: BCAT2 antibody at 14microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 34 kDa Observed band size: 34 kDa
Western blot All lanes: BCAT2 antibody at 14microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 34 kDa Observed band size: 34 kDa

BCAT2 Antibody

CSB-PA002601ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBCAT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    BCAT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID587
  • Target name
    BCAT2
  • Target description
    branched chain amino acid transaminase 2
  • Target synonyms
    BCAM; BCATM; BCT2; branched chain amino-acid transaminase 2, mitochondrial; branched chain aminotransferase 2, mitochondrial; branched-chain-amino-acid aminotransferase, mitochondrial; HVLI; placental protein 18; PP18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15382
  • Protein Name
    Branched-chain-amino-acid aminotransferase, mitochondrial
  • Scientific Description
    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCAT2 using anti-BCAT2 antibody (A06369-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT2 antigen affinity purified polyclonal antibody (Catalog # A06369-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAT2 at approximately 39 kDa. The expected band size for BCAT2 is at 44, 38-42 kDa.
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