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Chemical Structure
Chemical Structure
Chemical Structure

BCECF-AM [117464-70-7]

Research Use Only
CDX-C0003
Chemodex
CAS Number117464-70-7
Product group Chemicals
Estimated Purity>95%
Molecular Weight808.69
Price on request
Packing Size
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Overview

  • Supplier
    Chemodex
  • Product Name
    BCECF-AM [117464-70-7]
  • Delivery Days Customer
    10
  • CAS Number
    117464-70-7
  • Certification
    Research Use Only
  • Estimated Purity
    >95%
  • Hazard Information
    Non-hazardous,Warning
  • Molecular Formula
    C42H40O21
  • Molecular Weight
    808.69
  • Scientific Description
    Chemical. CAS: 117464-70-7. Formula: C42H40O21. MW: 808.69. Synthetic. Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: lambdaex 490nm; lambdaem 535nm; lambdaex=505nm; lambdaem=520nm in DMSO. - Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: lambdaex 490nm; lambdaem 535nm; lambdaex=505nm; lambdaem=520nm in DMSO.
  • SMILES
    CC(=O)OCOC(=O)CCC1=CC2=C(OC3=CC(OCOC(C)=O)=C(CCC(=O)OCOC(C)=O)C=C3C22OC(=O)C3=C2C=CC(=C3)C(=O)OCOC(C)=O)C=C1OCOC(C)=O
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    41116134