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Western blot analysis of extracts of HCT116 cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000. HCT116 cells treated with different concentRations of B7-H4.
Western blot analysis of extracts of HCT116 cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000. HCT116 cells treated with different concentRations of B7-H4.
Western blot analysis of extracts of HCT116 cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000. HCT116 cells treated with different concentRations of B7-H4.

BCL2 Polyclonal Antibody

E-AB-64067
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    BCL2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCL2 using anti-BCL2 antibody (A00040-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 antigen affinity purified polyclonal antibody (Catalog # A00040-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2 at approximately 26 kDa. The expected band size for BCL2 is at 26 kDa.
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