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BCL2A1 Antibody (aa60-69)

LS-C139653
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBCL2A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCL2A1 Antibody (aa60-69)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 20kD band observed in Human Bone Marrow lysates (calculated MW of 20.1kD according to NP_004040.1). Recommended concentration: 0.1-0.3 ug/ml.. Peptide-ELISA (1:8000), WB (0.1 - 0.3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 20kD band observed in Human Bone Marrow lysates (calculated MW of 20.1kD according to NP_004040.1). Recommended concentration: 0.1-0.3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID597
  • Target name
    BCL2A1
  • Target description
    BCL2 related protein A1
  • Target synonyms
    ACC1; ACC-1; ACC2; ACC-2; BCL2L5; bcl2-L-5; bcl-2-like protein 5; bcl-2-related protein A1; BFL1; GRS; HBPA1; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein; protein BFL-1
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCL2A1 using anti-BCL2A1 antibody (M03850). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2A1 antigen affinity purified monoclonal antibody (Catalog # M03850) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2A1 at approximately 27 kDa. The expected band size for BCL2A1 is at 20 kDa.
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