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ProductsBack/BCL2L11 / BIM Antibody (Preservative Free)

BCL2L11 / BIM Antibody (Preservative Free)

LS-C149230
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBCL2L11
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCL2L11 / BIM Antibody (Preservative Free)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:32000), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10018
  • Target name
    BCL2L11
  • Target description
    BCL2 like 11
  • Target synonyms
    BAM; bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; BCL2-like 11 (apoptosis facilitator); bcl-2-like protein 11; bcl-2-related ovarian death agonist; BIM; BOD
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.
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