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BCL2L2 Antibody (YA4406, Mouse)

HY-P84709
MedChem Express
TargetBCL2L2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BCL2L2 Antibody (YA4406, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID599
  • Target name
    BCL2L2
  • Target description
    BCL2 like 2
  • Target synonyms
    BCL-W, BCL2-L-2, BCLW, PPP1R51, bcl-2-like protein 2, apoptosis regulator BCL-W, protein phosphatase 1, regulatory subunit 51
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    BCL2L2 Antibody (YA4406) is a mouse-derived and non-conjugated IgG2a antibody, targeting to BCL2L2. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody (PA1426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Hela whole cell lysates., Lane 5: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl2L2 antigen affinity purified polyclonal antibody (Catalog # PA1426-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl2L2 at approximately 21KD. The expected band size for Bcl2L2 is at 21KD.
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