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Western Blot analysis of HuvEc cells using Bcr Polyclonal Antibody
Western Blot analysis of HuvEc cells using Bcr Polyclonal Antibody
Western Blot analysis of HuvEc cells using Bcr Polyclonal Antibody

BCR Antibody

CSB-PA000989
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBCR
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Overview

  • Supplier
    Cusabio
  • Product Name
    BCR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID613
  • Target name
    BCR
  • Target description
    BCR activator of RhoGEF and GTPase
  • Target synonyms
    ALL; BCR, RhoGEF and GTPase activating protein; BCR/FGFR1 chimera protein; BCR1; breakpoint cluster region; breakpoint cluster region protein; CML; D22S11; D22S662; FGFR1/BCR chimera protein; PHL; renal carcinoma antigen NY-REN-26
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11274
  • Protein Name
    Breakpoint cluster region protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCR using anti-BCR antibody (A00022-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCR antigen affinity purified polyclonal antibody (Catalog # A00022-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCR at approximately 160 kDa. The expected band size for BCR is at 160 kDa.
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