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ProductsBack/BEST1 / BEST / Bestrophin Antibody

BEST1 / BEST / Bestrophin Antibody

LS-C749082
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetBEST1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BEST1 / BEST / Bestrophin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 57kDa/67kDa/69kDa, while the observed MW by Western blot was 68kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 57kDa/67kDa/69kDa, while the observed MW by Western blot was 68kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7439
  • Target name
    BEST1
  • Target description
    bestrophin 1
  • Target synonyms
    ARB, BEST, BMD, Best1V1Delta2, RP50, TU15B, VMD2, bestrophin-1, Best disease, vitelliform macular dystrophy protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bestrophin using anti-Bestrophin antibody (A01434-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bestrophin antigen affinity purified polyclonal antibody (Catalog # A01434-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bestrophin at approximately 68KD. The expected band size for Bestrophin is at 68KD.
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