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ProductsBack/beta Amyloid 1-42 Polyclonal Antibody
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-beta-Amyloid(1-42) Polyclonal Antibody (bs-0107R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-beta-Amyloid(1-42) Polyclonal Antibody (bs-0107R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-beta-Amyloid(1-42) Polyclonal Antibody (bs-0107R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

beta Amyloid 1-42 Polyclonal Antibody

BS-0107R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAPP
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Overview

  • Supplier
    Bioss
  • Product Name
    beta Amyloid 1-42 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID351
  • Target name
    APP
  • Target description
    amyloid beta precursor protein
  • Target synonyms
    AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, amyloid-beta precursor protein, alzheimer disease amyloid A4 protein homolog, alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, amyloid beta A4 protein, amyloid precursor protein, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, cerebral vascular amyloid peptide, peptidase nexin-II, protease nexin-II, testicular tissue protein Li 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05067
  • Protein Name
    Amyloid-beta precursor protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chen YC, Chiu YJ, Lin CH, et al. Indole Compound NC009-1 Augments APOE and TRKA in Alzheimer's Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. J Alzheimers Dis. 2019,67(2):737-756. doi: 10.3233/JAD-180643
    Read this paper
  • Chen L, Yu P, Zhang L, et al. Methamphetamine exposure induces neuropathic protein β-Amyloid expression. Toxicol In Vitro. 2019,54:304-309. doi: 10.1016/j.tiv.2018.10.012
    Read this paper
  • Vinothkumar G, Kedharnath C, Krishnakumar S, et al. Abnormal amyloid β(42) expression and increased oxidative stress in plasma of CKD patients with cognitive dysfunction: A small scale case control study comparison with Alzheimer's disease. BBA Clin. 2017,8:20-27. doi: 10.1016/j.bbacli.2017.06.001
    Read this paper
  • Behairi N, Belkhelfa M, Rafa H, et al. All-trans retinoic acid (ATRA) prevents lipopolysaccharide-induced neuroinflammation, amyloidogenesis and memory impairment in aged rats. J Neuroimmunol. 2016,300:21-29. doi: 10.1016/j.jneuroim.2016.10.004
    Read this paper
  • Kang L, Li S, Xing Z, et al. Dihydrotestosterone treatment delays the conversion from mild cognitive impairment to Alzheimer's disease in SAMP8 mice. Horm Behav. 2014,65(5):505-15. doi: 10.1016/j.yhbeh.2014.03.017
    Read this paper
  • Zhang Q, Gao T, Luo Y, et al. Transient focal cerebral ischemia/reperfusion induces early and chronic axonal changes in rats: its importance for the risk of Alzheimer's disease. PLoS One. 2012,7(3):e33722. doi: 10.1371/journal.pone.0033722
    Read this paper
  • Xiao AW, He J, Wang Q, et al. The origin and development of plaques and phosphorylated tau are associated with axonopathy in Alzheimer's disease. Neurosci Bull. 2011,27(5):287-99. doi: 10.1007/s12264-011-1736-7
    Read this paper

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Figure 1. Western blot analysis of APP/C99 using anti-APP/C99 antibody (A00081-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP/C99 antigen affinity purified polyclonal antibody (Catalog # A00081-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP/C99 at approximately 99KD. The expected band size for APP/C99 is at 87KD.
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