
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631830) diluted at a dilution of 1:1000.
beta Tubulin 3/ Tuj1 antibody [GT886]
GTX631830
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTUBB3
Overview
- SupplierGeneTex
- Product Namebeta Tubulin 3/ Tuj1 antibody [GT886]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT886
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10381
- Target nameTUBB3
- Target descriptiontubulin beta 3 class III
- Target synonymsCDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta-3 chain, class III beta-tubulin, tubulin beta-4 chain, tubulin beta-III, tubulin, beta 3
- HostMouse
- IsotypeIgG1
- Protein IDQ13509
- Protein NameTubulin beta-3 chain
- Scientific DescriptionThis gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![beta III Tubulin antibody [GT886] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000. Red: Sox2 antibody (GTX101506) diluted at 1:250. beta III Tubulin antibody [GT886] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis. Sample:Frozen section of embryonic mouse brain (mE18.5). Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000. Red: Sox2 antibody (GTX101506) diluted at 1:250.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20150625_IHC_M_w_23061202_283.webp)
![Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20170309_WB_KO_watermark_w_23061202_404.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20161208_WB_M_w_23061202_430.webp)
![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000. Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20161208_WB_M_brain_w_23061202_943.webp)
![beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20170503_IFA_R_w_23061202_796.webp)
![beta Tubulin 3/ TUJ1 antibody [GT886] detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT886] (GTX631830) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). beta Tubulin 3/ TUJ1 antibody [GT886] detects beta Tubulin 3/ TUJ1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT886] (GTX631830) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX129913) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20160921_IHC-Fr_w_23061202_466.webp)
![beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250. beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20180817_IHC-P_R_w_23061202_330.webp)
![Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT886] (GTX631830). Western blot analysis was performed using beta III Tubulin antibody [GT886] (GTX631830). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent. Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT886] (GTX631830). Western blot analysis was performed using beta III Tubulin antibody [GT886] (GTX631830). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_41820_20150625_IP_w_23061202_349.webp)
![beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX631830/GTX631830_45875_20250905_IHC-P_M_25091123_549.webp)






![ICC/IF analysis of Neuro2a cells using GTX27751 beta Tubulin 3/ Tuj1 antibody [TU-20]. Green : Primary antibody Blue : DAPI Diultion : 3μg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX27751/GTX27751_20191028_ICC-IF_1_w_23060722_961.webp)
