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WB analysis of rat liver lysate using GTX88988 BHMT antibody, C-term. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat liver lysate using GTX88988 BHMT antibody, C-term. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat liver lysate using GTX88988 BHMT antibody, C-term. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer

BHMT antibody, C-term

GTX88988
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBHMT
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Overview

  • Supplier
    GeneTex
  • Product Name
    BHMT antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.03-0.1microg/ml. IHC-P: 2-4microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID635
  • Target name
    BHMT
  • Target description
    betaine--homocysteine S-methyltransferase
  • Target synonyms
    BHMT1, HEL-S-61p, betaine--homocysteine S-methyltransferase 1, epididymis secretory sperm binding protein Li 61p, phi-crystallin
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ93088
  • Protein Name
    Betaine--homocysteine S-methyltransferase 1
  • Scientific Description
    This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of BHMT using anti-BHMT antibody (A06712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BHMT antigen affinity purified polyclonal antibody (Catalog # A06712-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BHMT at approximately 45KD. The expected band size for BHMT is at 45KD.
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