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BI-1 (430)

SC-52895
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTMBIM6
50 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    BI-1 (430)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7009
  • Target name
    TMBIM6
  • Target description
    transmembrane BAX inhibitor motif containing 6
  • Target synonyms
    BAXI1, BI-1, TEGT, bax inhibitor 1, testis enhanced gene transcript, testis-enhanced gene transcript protein, transmembrane BAX inhibitor motif-containing protein 6
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
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