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Western Blot analysis of COLO205 cells using NBK Polyclonal Antibody
Western Blot analysis of COLO205 cells using NBK Polyclonal Antibody
Western Blot analysis of COLO205 cells using NBK Polyclonal Antibody

BIK Antibody

CSB-PA003373
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBIK
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Overview

  • Supplier
    Cusabio
  • Product Name
    BIK Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID638
  • Target name
    BIK
  • Target description
    BCL2 interacting killer
  • Target synonyms
    apoptosis inducer NBK; apoptosis-inducing NBK; bcl-2-interacting killer; BCL2-interacting killer (apoptosis-inducing); BIP1; BP4; natural born killer; NBK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13323
  • Protein Name
    Bcl-2-interacting killer
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Bik using anti-Bik antibody (PB9755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Spleen Tissue Lysate, Lane 2: Rat Gaster Tissue Lysate, Lane 3: Rat Intestine Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: A549 Whole Cell Lysate, Lane 6: SKOV Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bik antigen affinity purified polyclonal antibody (Catalog # PB9755) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bik at approximately 22KD. The expected band size for Bik is at 22KD.
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