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Biotin-14-dCTP

Research Use Only
ORB533284
Biorbyt
Product group Molecular Biology
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Biotin-14-dCTP
  • Delivery Days Customer
    16
  • Application Supplier Note
    Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: lambdamax 272 nm, epsilon 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
  • Certification
    Research Use Only
  • Concentration
    1.0-1.1 mM
  • Formulation
    Liquid
  • Scientific Description
    Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dCTP or Biotin-16-dCTP are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/50% dCTP.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352200