Biotin-14-dCTP

Biorbyt

Biotin-14-dCTP

16

Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: lambdamax 272 nm, epsilon 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

1.0-1.1 mM

Liquid

Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dCTP or Biotin-16-dCTP are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/50% dCTP.

-20°C

12352200