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BIRC3 / cIAP2 Antibody

LS-C812921
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBIRC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BIRC3 / cIAP2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID330
  • Target name
    BIRC3
  • Target description
    baculoviral IAP repeat containing 3
  • Target synonyms
    AIP1; API2; apoptosis inhibitor 2; baculoviral IAP repeat-containing protein 3; cellular inhibitor of apoptosis 2; CIAP2; c-IAP2; HAIP1; HIAP1; IAP homolog C; IAP-1; inhibitor of apoptosis protein 1; MALT2; mammalian IAP homolog C; MIHC; RING finger protein 49; RING-type E3 ubiquitin transferase BIRC3; RNF49; TNFR2-TRAF signaling complex protein; TNFR2-TRAF-signaling complex protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of cIAP2 using anti-cIAP2 antibody (PB9527). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HEPG2 Whole Cell Lysate at 40ug, Lane 2: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cIAP2 antigen affinity purified polyclonal antibody (Catalog # PB9527) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cIAP2 at approximately 68 kDa. The expected band size for cIAP2 is at 68 kDa.
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