Bispecific BCMA:CD3 Bridging Colorimetric ELISA Kit

BPS Bioscience

Bispecific BCMA:CD3 Bridging Colorimetric ELISA Kit

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Other Application

Research Use Only

The Bispecific BCMA:CD3 Bridging Colorimetric ELISA Kit is an ELISA designed to analyze the ability of Bispecific Antibodies (BsAbs) to bridge BCMA (B-Cell Maturation Antigen, also known as Tumor necrosis factor receptor superfamily member 17 (TNFRSF17)) and CD3 (cluster of differentiation 3) for screening and profiling applications. This assay kit can determine if an anti-BCMA-anti-CD3 bispecific antibody binds to both targets simultaneously and bridges BCMA to CD3. The Bispecific BCMA:CD3 Bridging Colorimetric ELISA Assay Kit comes with enough recombinant human BCMA (amino acids 20-291), CD3- Containing Detection Reagent, and assay buffer for 100 enzyme reactions. This kit also includes Anti-BCMA-Anti-CD3 Bispecific Antibody as a positive . A 96-well plate is coated with BCMA protein. After coating, an anti-BCMA-anti-CD3 bispecific antibody is added in an optimized assay buffer. The plate is washed to remove the unbound antibody. The plate is then incubated with CD3-Containing Detection Reagent. Finally, HRP Colorimetric Substrate is added to produce absorbance that can be measured using a UV/Vis spectrophotometer microplate reader. The absorbance signal is proportional to the bridging efficacy. Need us to run inhibitor screens or profile your bispecific antibodies towards CD3 and BCMA? Check out our Immunotherapy Biochemical Screening Services.

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