Bispecific Mesothelin:CD3 Bridging Colorimetric ELISA Kit

BPS Bioscience

Bispecific Mesothelin:CD3 Bridging Colorimetric ELISA Kit

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Other Application

Research Use Only

The Bispecific Mesothelin:CD3 Bridging Colorimetric ELISA Kit is an ELISA designed to analyze the ability of Bispecific Antibodies (BsAbs) to bridge Mesothelin (also known as MSLN, MPF, SMRP) and CD3 (cluster of differentiation 3) for screening and profiling applications. This assay kit can determine if an anti-Mesothelin-anti-CD3 bispecific antibody binds to both targets simultaneously and bridges Mesothelin to CD3. The Bispecific Mesothelin:CD3 Bridging Colorimetric ELISA Assay Kit comes with enough recombinant human Mesothelin (amino acids 296-580), CD3-Containing Detection Reagent, and assay buffer for 100 enzyme reactions. This kit also includes Anti-Mesothelin-Anti-CD3 Bispecific Antibody as a positive control. A 96-well plate is coated with Mesothelin protein. After coating, an anti-Mesothelin-anti-CD3 bispecific antibody is added in an optimized assay buffer. The plate is washed to remove the unbound antibody. The plate is then incubated with CD3-Containing Detection Reagent. Finally, HRP Colorimetric Substrate is added to produce absorbance that can be measured using a UV/Vis spectrophotometer microplate reader. The absorbance signal is proportional to the bridging efficacy.

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